Phagocytosis

Phagocytosis (fromAncient Greekφαγεῖν (phagein) 'to eat'andκύτος (kytos) 'cell') is the process by which a cell uses its plasma membrane to engulf a large particle (≥ 0.5 μm), giving rise to an internal compartment called the phagosome. It is one type of endocytosis. A cell that performs phagocytosis is called a phagocyte.

In a multicellular organism'simmune system, phagocytosis is a major mechanism used to remove pathogens and cell debris. The ingested material is then digested in the phagosome. Bacteria, dead tissue cells, and small mineral particles are all examples of objects that may be phagocytized. Some protozoa use phagocytosis as means to obtain nutrients. The two main cells that do this are the Macrophages and the Neutrophils of the immune system.

Where phagocytosis is used as a means of feeding and provides the organism part or all of its nourishment, it is called phagotrophy and is distinguished from osmotrophy, which is nutrition taking place by absorption.^[1]

The history of phagocytosis represents the scientific establishment of immunology as the process is the first immune response mechanism discovered and understood as such.^[2][3] The earliest definitive account of cell eating was given by Swiss scientist Albert von Kölliker in 1849.^[4] In his report in Zeitschrift für Wissenschaftliche Zoologie, Kölliker described the feeding process of an amoeba-like alga, Actinophyrys sol (a heliozoan) mentioning details of how the protist engulfed and swallowed (the process now called endocytosis) a small organism, that he named infusoria (a generic name for microbes at the time).^[5]

The first demonstration of phagocytosis as a property of leucocytes, the immune cells, was from the German zoologist Ernst Haeckel.^[6][7] Haeckel discovered that blood cells of sea slug, Tethys, could ingest Indian ink (or indigo^[8]) particles. It was the first direct evidence of phagocytosis by immune cells.^[6][8] Haeckel reported his experiment in a 1862 monograph Die Radiolarien (Rhizopoda Radiaria): Eine Monographie.^[9]

Phagocytosis was noted by Canadian physician William Osler (1876),^[10] and later studied and named by Élie Metchnikoff (1880, 1883).^[11]

Phagocytosis is one main mechanisms of the innate immune defense. It is one of the first processes responding to infection, and is also one of the initiating branches of an adaptive immune response. Although most cells are capable of phagocytosis, some cell types perform it as part of their main function. These are called 'professional phagocytes.' Phagocytosis is old in evolutionary terms, being present even in invertebrates.^[12]

Following apoptosis, the dying cells need to be taken up into the surrounding tissues by macrophages in a process called efferocytosis. One of the features of an apoptotic cell is the presentation of a variety of intracellular molecules on the cell surface, such as calreticulin, phosphatidylserine (from the inner layer of the plasma membrane), annexin A1, oxidised LDL and altered glycans.^[21] These molecules are recognised by receptors on the cell surface of the macrophage such as the phosphatidylserine receptor or by soluble (free-floating) receptors such as thrombospondin 1, GAS6, and MFGE8, which themselves then bind to other receptors on the macrophage such as CD36 and alpha-v beta-3 integrin. Defects in apoptotic cell clearance is usually associated with impaired phagocytosis of macrophages. Accumulation of apoptotic cell remnants often causes autoimmune disorders; thus pharmacological potentiation of phagocytosis has a medical potential in treatment of certain forms of autoimmune disorders.^{[22][23][24][25]}

Phagocytosis is used by many protists as a means of feeding, thus constituting phagotrophy.

• In some, such as amoeba, phagocytosis takes place by surrounding the target object with pseudopods, as in animal phagocytes. In humans, the amoebozoan Entamoeba histolytica can phagocytose red blood cells.
• Ciliates also engage in phagocytosis.^[26] In ciliates there is a specialized groove or chamber in the cell where phagocytosis takes place, called the cytostome or mouth.

As in phagocytic immune cells, the resulting phagosome may be merged with lysosomes (food vacuoles) containing digestive enzymes, forming a phagolysosome. The food particles will then be digested, and the released nutrients are diffused or transported into the cytosol for use in other metabolic processes.^[27]

Mixotrophy can involve phagotrophic nutrition and phototrophic nutrition.^[28]

• Media related to Phagocytosis at Wikimedia Commons
• Phagocytosis at the U.S. National Library of Medicine Medical Subject Headings (MeSH)